Does his absence make a difference to your work?

没有他，会影响你的工作吗？

　　make a/no/some difference to sb./sth.: have an/no/some effect on sb./sth. 对……有效、无效、有些效果；对……重要、不重要
　　例句：
　　Whether he is here or not will make no difference to our work.
　　他在不在对我们的工作没有影响。
　　Education can make a big difference to the quality of a person's life.
　　教育对一个人的生活质量有很大影响。