please表达接受帮助：
Would you like a cup of coffee？Yes, please.
要不要來一杯咖啡。好啊！
May I help you？Yes, please. I'd like to...
需要帮忙吗？好啊，我想要……

please表达请求：
Could you give me a hand, please？
可以帮我忙吗？
May I use your pen, please？
我可以用你的笔吗？

And we can also use "could"、"would":
Could you possibly send me the file？
Would you mind sending me the file？
Would you be able to send me the file？
可以寄档案給我吗？
Could I have it within ten minutes please？
我可以10分钟内收到档案吗？